Adsorption of Human Immunoglobulin G to Poly (β-hydroxybutyrate) (phb), Poly (l- Lactic Acid) (plla) and Phb/plla Blends
نویسندگان
چکیده
Biodegradable polymeric materials can be used as temporary implants and may be able to carry out specific functions for a pre-determined period prior to their degradation in vivo. In order to be used they must present characteristics of biocompatibility. When a material comes into contact with blood, the instantaneous adsorption of protein occurs on its surface. Coverage of the surface by γ-globulins causes the adhesion of platelets and, consequently, provokes the process of blood coagulation. In this context, the objective of the present paper was to quantify the adsorption of human Immunoglobulin G (HIgG) to poly (β-hydroxybutyrate) (PHB), poly (L-lactic acid) (PLLA) and PHB/PLLA blends using the FTIR/ATR technique. The results showed the occurrence of HlgG adsorption for all three systems, PHB having the highest adsorption density, about Γmax =2.57μg/cm, followed by PHB/PLLA blends (Γmax=0.85μg/cm) and PLLA with the lowest values (Γmax=0.25μg/cm).
منابع مشابه
Evaluation of homogeneity of binary blends of poly(3-hydroxybutyrate) and poly(L-lactic acid) studied by near infrared chemical imaging (NIRCI).
The homogeneity of blends of poly((R)-3-hydroxybutyrate) (PHB) and poly(L-lactic acid) (PLLA) was evaluated by the near infrared chemical imaging (NIRCI) technique. NIRCI can nondestructively investigate a sample over a wide field of view within a few minutes to acquire a large number of spatially resolved NIR spectral data. NIRCI may be combined with multivariate analysis not only for qualitat...
متن کاملThe Effect of Additives Interaction on the Miscibility and Crystal Structure of Two Immiscible Biodegradable Polymers
Poly lactic acid (PLLA) is a promising biopolymer, obtained from polymerization of lactic acid that is derived from renewable resources through fermentation. The characteristic brittleness of PLLA is attributed to slow crystallization rates, which results in the formation of the large spherulites. Its glass temperature is relative high, above room temperature and close to 60 °C, and therefore i...
متن کاملImmunostimulatory and growth-promoting potential of poly-β-hydroxybutyrate in rainbow trout (Oncorhynchus mykiss) fingerlings culture
In the current study, the effect of dietary supplementation of poly-β-hydroxybutyrate (PHB) on growth performance, digestive enzymes activity, body composition, non-specific immune response and diseases resistance of rainbow trout fingerlings were investigated. Five hundred and fifty-two healthy fingerlings (8.2±1.0 g) were randomly distributed in 12 tanks (150 L) at a density of 46 fish/ tank ...
متن کاملEffects of natural polymer (Poly-β-hydroxybutyrate) and its degrading bacteria on lipid metabolism and digestive tract pH in Siberian Sturgeon Acipenser baerii Brandt, 1869 fingerlings
Five hundred and ninety two Siberian sturgeon (A. baerii) fingerlings (average weight, 11.1±0.9 g) were randomly distributed in 16 tanks (150 L) at a density of 35 fish per tank and fed different treatments (Control, degrading bacteria, 2% PHB, degrading bacteria + 2%PHB) for 60 days. At the end of the experiment, the results indicated that the fish fed PHB or bacteria treatments had low midgut...
متن کاملIn vitro and in vivo degradation studies for development of a biodegradable patch based on poly(3-hydroxybutyrate).
For the development of a resorbable gastrointestinal patch, the in vitro degradation of solution-cast films of poly(3-hydroxybutyrate) (PHB), modifications of PHB expected to influence its degradation time, as well a poly(L-lactide) (PLLA) was examined. The molecular weight of pure PHB decreased by one-half after 1 year in buffer solution (pH 7.4, 37 degrees C). Acceleration in molecular weight...
متن کامل